Abstract
Cysticercosis is a neglected tropical disease caused by the larvae of Taenia solium in pigs and humans. The current diagnosis of porcine cysticercosis is difficult, and traditional pathological tests cannot meet the needs of detection. This study established a UPT-LF assay for the detection of Cysticercus cellulosae. UCP particles were bound to two antigens, TSOL18 and GP50; samples were captured, and the signal from the UCP particles was converted into a detectable signal for analysis using a biosensor. Compared to ELISA, UPT-LF has higher sensitivity and specificity, with a sensitivity of 93.59% and 97.44%, respectively, in the case of TSOL18 and GP50 antigens and a specificity of 100% for both. Given its rapidness, small volume, high sensitivity and specificity, and good stability and reproducibility, this method could be used in the diagnosis of cysticercosis.
Highlights
Cysticercosis is an important zoonotic parasitic disease, which is mainly caused by eating Taenia solium larvae by mistake. (Bizhani et al, 2020)
Several positive sera were detected by UPT-LF to evaluate the specificity, including sera containing T. asiatica, T. gondii, C. sinensis, and T. spiralis
The UPT-LF assay system consists of a sample dilutant, a test card, and a biosensor (Figure 1)
Summary
Cysticercosis is an important zoonotic parasitic disease, which is mainly caused by eating Taenia solium larvae by mistake. (Bizhani et al, 2020). The serum to be tested was diluted with 1% BSA and added to a 96-well enzyme reaction plate. When the value of the sample to be tested was more than 2.1 times the negative control value, it was judged to be positive. Sensitivity, Specificity, and Stability Test of the UPT-LF Assay
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