Abstract
The ligated intestinal loop technique in rabbits for the assay of Clostridium perfringens enterotoxin was improved by decreasing the incubation time from about 6 h to 90 min, and keeping the animal under anaesthesia during this period.The fluid volumes were significantly higher in loops injected with enterotoxin than in loops injected either with saline or with a mixture of enterotoxin and specific antiserum. These differences were mainly due to a net loss of fluid from the control loops.
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