Rapid Detection of Candida species Isolated from Denture Stomatitis Patients using Phenotypic methods and Chromogenic agar media

Hayffaa Abdulla,Eman Mustafa

doi:10.33899/rden.2020.126821.1029

Abstract

Aims: The purpose of this study was Isolation and phenotypic identification of different candida species isolated from denture stomatitis patients. Materials and Methods : Over all 62 isolates of Candida species yielded from 43 patients attending prosthodontic departmentCollege of DentistryMosul universityDental teaching Hospital. Clinically, the samples processed by traditional methods including culture characteristics, gram staining reaction , germ tube, chlamydospore formation, culture on CHROM agar Candida , Sabouraud Dextrose agar, urea's test and Carbohydrates fermentation test. Results: All isolates were accurately diagnosed to the species level by various traditional methods , among the total of 62 Candida species , the predominant type was the Candida albicans which accounted for (46.8%) followed by Candida glabrata (33.9%), Candida tropicalis (17.7%), finally the species Candida krusei accounted for (1.6%). Conclusions: Candida albicans is highly spreading among patients wearing dentures particularly those suffering from denture stomatitis in which CHROM agar Candida medium is best method for rapid identification and documentation of different Candida species .

Highlights

Candida species are regarded the most common isolated and the frequent etiological factor of denture stomatitis. (1) Approximately 300 Candida species are documented,10 % can cause infections in human(2)
Different species of Candida are described as a communal microorganisms, they exist in 30-60 % of healthy people and 60-100% of patient with denture stomatitis (3,4), non albicans species including Candida glabrata, Candida tropicalis, Candida parapsilosis, and Candida krusei etc . appeared as etiological agents in oral candidiosis either as a co-infection with C.albicans or as a sole pathogenic microorganism(4,5). there are different conventional methods for identification of yeast such as: culture on sabouraud dextrose agar (SDA) media,corn meal agar ( CMA), germ tube test, carbohydrate fermentation test,and in order to accelerate detection of candida CHROM agar are used as a selective and differential media which inhibit the growth of any microorganisms except candida (6)
To differentiate between C. albicans from non albicans groups, human blood was Denture Stomatitis Patients placed in test tube and left in rack to clot, after clotting the sample centrifuged at 3000 rpm for 10 min, serum was separated carefully and placed in sterile test tube and colony was picked up with sterile loop and was placed in 0.5 ml of serum, this mixture was placed in an incubator at 37 C0 for [3,4] hrs

Summary

INTRODUCTION

Candida species are regarded the most common isolated and the frequent etiological factor of denture stomatitis. (1) Approximately 300 Candida species are documented ,10 % can cause infections in human(2). To differentiate between C. albicans from non albicans groups, human blood was Denture Stomatitis Patients placed in test tube and left in rack to clot, after clotting the sample centrifuged at 3000 rpm for 10 min, serum was separated carefully and placed in sterile test tube and colony was picked up with sterile loop and was placed in 0.5 ml of serum, this mixture was placed in an incubator at 37 C0 for [3,4] hrs Adrop of this mixture was placed , put on a clean slid, overlaid with a clean cover slip and examined by high power objective lens of microscope (40x) (7) C- Chlamydospor production : The colony was picked up from selective media and was cultured on 1cm x shape in corn meal agar, covered with sterile cover slip the plate incubated at room temperature for [48-72] hrs. Twenty nine species of C.albicans produce chlamydospore and only two species of C.tropicalis gave positive result (Table[1])

Positive Negative

Pink with white borders rough colonies

DISCUSSION

Findings

CONCLUSIONS