Abstract
Diagnosis of infections with BRSV have previously been limited to histopathologic descriptions of atypical interstitial pneumonia with microscopic lesions of large, multinucleated giant cells and syncytial cells in the lung. False negative fluorescent antibody (FA) tests on tissue sections may be a result of a viral infection of only superficial bronchial cells. Virus isolation is even a less successful method of identification than FA with a report in one study of 1.7% positive isolations. Most isolates require numerous subpassaging before a cytopathic effect is noticed, but even after numerous passages, the viral titers remain low. Another method for identifying current infections with BRSV is the indirect fluorescent antibody on paired sera to detect a rise in antibody titer. New rapid assays for diagnosing human respiratory syncytial virus have been compared to the slower and less sensitive conventional methods of serology and viral isolation.
 The enzyme immunoassays have been found to be a highly sensitive and specific technique for the identification of human respiratory syncytial virus antigens. The antigenic similarities between bovine and human respiratory syncytial virus allowed the use of the human assay for the detection of the bovine virus. The Oklahoma Animal Disease Diagnostic Laboratory evaluated the Abbott TestPack® RSV, which requires less than 30 minutes, and compared it to the slower determinations of virology, necropsy, histopathology and bacteriology.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.