Abstract
Coronavirus disease 2019 is an infectious disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). SARS-CoV-2 is highly transmissible. Early and rapid testing is necessary to effectively prevent and control the outbreak. Detection of SARS-CoV-2 antibodies with lateral flow immunoassay can achieve this goal. In this study, SARS-CoV-2 nucleoprotein (NP) was expressed and purified. We used the selenium nanoparticle as the labeling probe coupled with the NP to prepare an antibody (IgM and IgG) detection kit. The detection limit, cross reaction, sensitivity and specificity of the kit is verified. Separate detection of IgM and IgG, such as in this assay, was performed in order to reduce mutual interference and improve the accuracy of the test results.The final purity of NP was 91.83%. Selenium nanoparticle and NP successfully combined with stable effect. The LOD of the kit was 20 ng/mL for anti-NP IgG and 60 ng/mL for anti-NP IgM, respectively. The kit does not cross reaction with RF. The sensitivity of the kit was 94.74% and the specificity was 96.23%. The assay kit does not require any special device for reading the results and the readout is a simple color change that can be evaluated with the naked eye. This kit is suitable for rapid and real-time detection of the SARS-CoV-2 antibody IgG and IgM.
Highlights
B EGINNING in December 2019, several unexplained pneumonia cases occurred in Wuhan City, Hubei Province
Chinese researchers identified the cause of the disease as a new type of coronavirus, namely severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) [1]
SARS-CoV-2 contains spike protein, envelope protein, membrane protein, and nucleoprotein
Summary
B EGINNING in December 2019, several unexplained pneumonia cases occurred in Wuhan City, Hubei Province. Chinese researchers identified the cause of the disease as a new type of coronavirus, namely severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) [1]. SARS-CoV-2 contains spike protein, envelope protein, membrane protein, and nucleoprotein. Of these proteins, nucleoprotein is the most highly conserved [3]. More than 210 countries and regions have reported confirmed cases of the coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2. SARS-CoV-2 has a long incubation period, a wide range of transmission channels, and screening is required for a large population, all of which increases the difficulty in creating an optimal detection assay.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
