Abstract
The accumulation of β-amyloid (Aβ) is the earliest event seen in the neocortex and hippocampus of Alzheimer's disease (AD) patients. Transgenic mouse models of Aβ deposition are excellent tools for validating pharmacological therapies for reducing Aβ burden. Sensitive and rapid probes should be needed for detecting Aβ plaques ex vivo and in vivo in the transgenic mouse models. However, a thioflavin derivative, Pittsburgh Compound-B (PIB), which is a successful PET tracer for detecting Aβ plaques in AD brains, does not visualize Aβ plaques in APP and PS1/APP transgenic mice. Here, we report that Hoechst 33342, a cell-permeable fluorescent probe for staining DNA and nuclei, also detects Aβ plaques in APP Tg mouse. These findings could allow us to rapidly detect Aβ plaques in AD mouse models, and to develop improved compounds for detecting Aβ plaques in vivo in mouse models.
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