Abstract
The rapid analysis of 6-benzylaminopurine (6-BAP) residue by surface-enhanced Raman spectroscopy with a portable Raman spectrometer is reported. The results showed that the Raman signals of 6-BAP can be significantly enhanced when mixed with a gold nanoparticle colloid substrate. The typical Raman shifts of 6-BAP extraction were at 1002, 1318, and 1336 cm–1 and the intensities of normalized characteristic band at 1002 cm–1 (I1002 to I738) showed high correlation with 6-BAP concentrations. The concentration linear range was 0.1–5.0 μg/mL. The coefficient of determination (R2) was about 0.99, and calculated RSDs below 10%. The SERS data matched well with the HPLC results.
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