Abstract
Three pathogens,N. meningitidis,H. influenzae, andS. pneumoniae, have been successfully detected and identified in clinical cerebrospinal fluids using SERS method.
Highlights
Surface-enhanced Raman scattering (SERS) spectroscopy has recently received increasing attention due to its huge potential in the highly selective and sensitive detection of various types of molecules, even at a single-molecule detection level.[1,2] SERS, in which the scattering cross-section is considerably enhanced for molecules upon their adsorption onto metallic nanostructures, Paper exhibit a uniform and high enhancement factor (EF), chemical stability, and a cheap and reproducible production method
The level of neopterin was signi cantly higher in cerebrospinal fluid (CSF) samples infected by N. meningitidis, (30 nmol LÀ1), S. pneumoniae (38 nmol LÀ1), H. in uenzae (32 nmol LÀ1) compared to normal CSF samples (3.8 nmol LÀ1)
The results of our study clearly demonstrate that: (i) neopterin may be used as a novel marker in the screening tests to detect bacterial meningitis and (ii) in the positive response, the simultaneous identi cation of three of the most common meningitis pathogens, e.g., Neisseria meningitidis, Haemophilus in uenzae, and Streptococcus pneumoniae in clinical cerebrospinal uid sample is possible using our new class of SERS substrates
Summary
Three of the most common meningitis pathogens, Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae, have been successfully detected and identified in clinical cerebrospinal fluid (CSF) samples using a new class of a surface-enhanced Raman scattering (SERS) assay. The approach presented in this study challenges the current SERS-based method of microorganism detection in terms of sensitivity and, more importantly, reveals a simple, quick (on a timescale of seconds), label-free detection of multiple components from very small volumes of clinical samples. This new SERS class of assay, based on the combination of two types of Au/Ag-coated, nuclepore track-etched polycarbonate membranes, allow simultaneous filtration of CSF and immobilization of CSF components, enhancing their Raman signals and enabling detection of the spectra of a single bacteria cell present in the analyzed CSF samples. The neopterin concentration can be used to predict meningitis, but cannot be applied to qualify the species of bacteria inducing the meningitis infection
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