Abstract
Rapid detection of pathogens and assessment of antimicrobial susceptibility is of great importance for public health, especially in resource-limiting regions. Herein, we developed a rapid, portable, and universal detection method for bacteria using AgNPs-invertase complexes and the personal glucose meter (PGM). In the presence of bacteria, the invertase could be released from AgNPs-invertase complexes where its enzyme activity of invertase was inhibited. Then, the enzyme activity of invertase was restored and could convert sucrose into glucose measured by a commercially PGM. There was a good linear relationship between PGM signal and concentration of E. coli or S. aureus as the bacteria model with high sensitivity. And our proposed biosensor was proved to be a rapid and reliable method for antimicrobial susceptibility testing within 4 h with consistent results of Minimum Inhibitory Concentrations (MICs) testing, providing a portable and convenient method to treat infected patients with correct antibiotics and reduce the production of antibiotic-resistant bacteria, especially for resource-limiting settings.
Highlights
Rapid detection of pathogens and assessment of antimicrobial susceptibility is of great importance for the treatment of infectious diseases, especially in resource-limiting regions
The biosensor combines three main components: 1) invertase, an anionic enzyme, to provide signal amplification and convert sucrose into glucose measured by personal glucose meter (PGM); 2) sucrose, a catalytic substrate of invertase; 3) PEI-AgNPs, a cationic nanoparticle, which could recognize bacteria and reversibly bind to invertase, inhibiting the catalytic activity
The active invertase converts sucrose into glucose, which could be measured by a commercially PGM
Summary
Rapid detection of pathogens and assessment of antimicrobial susceptibility is of great importance for the treatment of infectious diseases, especially in resource-limiting regions. A few colorimetric and smell-based rapid bacteria detection methods based on nanoparticle-enzyme (e.g., β-galactosidase (β-Gal) (Miranda et al, 2011; Thiramanas and Laocharoensuk, 2016), lipase (Duncan et al, 2017), and urease (Singh et al, 2019) complexes have been reported. These methods are rather qualitative, and the quantitative assay is still requiring advanced instruments such as UV-vis spectrometry. The proof-of-concept experiments were conducted by measuring the concentration of E. coli after being treated with antibiotics
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