Abstract
A rapid, continuous, colorimetric enzyme assay for penicillin G acylase has been developed. The assay measures the formation of the acidic products of penicillin G hydrolysis by following the decrease in pH using Phenol Red as an indicator. The activity measured is directly proportional to the amount of enzyme added to the assay, having a linear relationship with an R 2 value of 0.9994.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have