Rapid cold-induced changes of membrane order and Δ 9-desaturase activity in endoplasmic reticulum of carp liver: A time-course study of thermal acclimation

Abstract

The membrane order of liver endoplasmic reticulum (ER) membranes of 10°C- and 30°C-acclimated carp has been compared using the fluorescence polarization technique with DPH as probe. Membranes from cold-acclimated fish displayed lower polarizations than corresponding membranes from warm-acclimated fish, the difference compensating for 34–50% of the direct effects of temperature upon polarization. The changes in Δ 9-desaturase activity and fluorescence polarization of DPH in ER membranes have been monitored as a function of time during cold acclimation of 30°C-acclimated carp. Cooling was achieved in three stages over 48 h. Desaturase activity in both rough and smooth ER showed a rapid increase in activity for the first three days followed by a decline on day 4 and a second increase up to day 10. Polarization of DPH (measured at 10°C) was rapidly reduced on cooling with no further change after day 4. The halftime for change in polarization and for the first desaturase induction were both approx. 2 days although large changes in polarization were evident within 24 h after the onset of cooling. During the cooling phases the daily changes in DPH polarization were quantitatively related to increments in desaturase capacity. The second desaturase induction had no effect upon membrane structure, at least as indicated by the polarization technique.