Rapid clonal propagation of Plumbago zeylanica Linn.

Abstract

An efficient protocol was developed for in vitro clonal propagation of Plumbago zeylanica Linn. through nodal culture. Multiple shoots were induced from nodal explants of P. zeylanica on Murashige and Skoog's (1962) medium supplemented with 0.5 mg L−1 to 1.0 mg.L−1 6-benzyladenine and 3% (w/v) sucrose. Inclusion of IAA (0.01 mg L−1) in the culture medium improved the frequency of production of multiple shoots. Rooting was readily achieved upon transferring the shoots onto half-strength MS medium supplemented with 0.25 mg L−1 IBA and 2% (w/v) sucrose. Micropropagated plantlets were hardened in the greenhouse and successfully established in soil.