Abstract
The notion to fight against multi drug resistant pathogens is a great deal in the field of nanomedicine. The identifiable antimicrobial action of metal bionanoparticles on many microorganisms is reported earlier. As silver bionanoparticles (Ag-BNPs) are known to have efficient antibacterial properties they are synthesized in ecofriendly and biocompatible way. The present study is focused on the extracellular biosynthesis of highly stable Ag-BNPs from bacterial strain Bacillus megaterium (NCIM 2326) by bio-reduction of silver ion using the culture supernatant, and to determine the antibacterial efficacy on multi drug resistant clinical pathogens such as Streptococcus pneumoniae and Salmonella typhi. The biosynthesis process is rapid and Ag-BNPs are formed within few minutes if AgNO(3) comes to contact with cell filtrate. Furthermore the synthesized Ag-BNPs are characterized by UV-vis spectroscopy, Atomic Force Microscopy (AFM), Thin Layer Chromatography (TLC) and Fourier Transform Infrared Spectroscopy (FTIR).
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