Abstract
The biosynthesis of nanoparticles is a cost effective, eco-friendly and non toxic alternative to physical and chemical methods. In the present study, we report a rapid and eco-friendly method for extracellular biosynthesis of gold nanoparticles (GNPs) using cell free extract of Staphylococcus epidermidis. In this method, the formation of GNPs is rapid as the reaction process completes within few minutes. The synthesis of GNPs was preliminary observed by colour change from pale yellow to pink red and confirmed by peaks at 545–550nm using a UV–visible spectroscopy. Transmission electron microscopy (TEM) showed formation of well dispersed GNPs in the range of 20–25nm. Fourier transform-infrared spectroscopy (FT-IR) was used to characterise the functional groups which might have played an important role in reduction and stabilisation of GNPs. The synthesised GNPs showed good catalytic activity for methylene blue degradation. This is the first to report on the rapid and ecofriendly method for the biosynthesis of GNPs by S. epidermidis.
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