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Abstract
Sensitive assays have been developed for adenosine 5'-triphosphate, the reduced form of nicotinamide adenine dinucleotide, cholyl glycine and alcohol, with immobilized and co-immobilized preparations of bacterial and firefly luciferase as reagents. With high-speed (ASA 20000) instant photographic film as detector, picomole amounts of the various analytes can be detected rapidly. The simplicity and convenience of the analytical combination of co-immobilized bioluminescent enzymes and photographic film for the detection of light make this an ideal technique for rapid screening tests.
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