Abstract
An optimization strategy for the separation and analysis of class III metallothioneins from a complex matrix by capillary zone electrophoresis (CZE) is presented and illustrated. Metallothioneins are a group of polypeptides used for many organisms to alleviate stresses imposed by exposure to heavy-metal excess. The analysis requires several steps: chromatography on exclusion or anion-exchange columns, ultrafiltration and HPLC with derivatization. In the present paper, a rapid and simple separation technique by CZE with an uncoated polyimide-clad fused-silica capillary is described. This technique permits the analysis of these molecules without prior steps of purification and derivatization. The running conditions were voltage −12 kV, room temperature and 150 mM sodium phosphate buffer (pH 3.5). Based on this preliminary study, CZE with an uncoated fused-silica capillary using sodium phosphate buffer appears to be a useful technique for the separation of class III metallothioneins.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
