Abstract
BackgroundGlaesserella parasuis (G. parasuis) is an influential pathogen of the pig, which induces high morbidity and mortality in naive pig populations in the pig industry. Accurate and rapid detection of the agent is important for disease control. In this study, a simple recombinase polymerase amplification (RPA) with a Lateral flow (LF) strip (RPA-LF-GPS) was developed to detect G. parasuis.ResultsThe RPA-LF-GPS can specifically detect G. parasuis a limit of 100 CFU from other common related pathogens causing arthritis in the pig. The RPA-LF-GPS assay can use boiled synovial fluid samples as a template with the same sensitivity as other DNA extraction methods. In the detection of clinic positive synovial fluid sample, RPA-LF-GPS is equally sensitive (98.1%) compared with that of PCR (90.4%) (P > 0.05). The whole procedure of the RPA-LF-GPS assay could be finished in 1 hour without professional equipment.ConclusionsRPA-LF-GPS assay is a rapid and simple method for point-of-care diagnostic testing for G. parasuis infection.
Highlights
Glaesserella parasuis (G. parasuis) is an influential pathogen of the pig, which induces high morbidity and mortality in naive pig populations in the pig industry
Glaesserella parasuis disease, known as Glaesser’s disease, is a common disease of pig caused by G. parasuis (GPS) previously known as Haemophilus parasuis, which is characterized by early colonization of the porcine upper respiratory tract [1] and difficult to be controlled by management procedures such as segregated early weaning
Development of recombinase polymerase amplification (RPA)-Lateral flow (LF)-GPS assay The RPA assay was performed under different temperature and time to optimize the reaction condition
Summary
Glaesserella parasuis (G. parasuis) is an influential pathogen of the pig, which induces high morbidity and mortality in naive pig populations in the pig industry. Results: The RPA-LF-GPS can detect G. parasuis a limit of 100 CFU from other common related pathogens causing arthritis in the pig. The RPA-LF-GPS assay can use boiled synovial fluid samples as a template with the same sensitivity as other DNA extraction methods. In the detection of clinic positive synovial fluid sample, RPA-LF-GPS is sensitive (98.1%) compared with that of PCR (90.4%) (P > 0.05). Glaesserella parasuis disease, known as Glaesser’s disease, is a common disease of pig caused by G. parasuis (GPS) previously known as Haemophilus parasuis, which is characterized by early colonization of the porcine upper respiratory tract [1] and difficult to be controlled by management procedures such as segregated early weaning. It is necessary to distinguish GPS infection from those caused by other pathogens allowing targeted treatment of the disease
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