Abstract
A lateral flow immunoassay (LFA) for rapid and sensitive quantitation of zearalenone (ZEN) in food and feed stuffs was developed, based on the availability of a new highly sensitive anti-ZEN monoclonal antibody. Compared to previously reported LFA, the established method allows quantitative determination of ZEN within the desirable dynamic range of 1–50 ng mL−1, corresponding to the recorded color intensity (CI) values of 65–9, with an IC50 value of 15 ng mL−1. The developed approach was then applied to a sample collection of thirty two cereal-based baby food, maize, wheat and feed samples, revealing that seven positive samples contained ZEN at concentrations ranging from 20 to 94 μg kg−1. The accuracy of the obtained results was confirmed by in-parallel LC–MS/MS analyses. The described assay format is capable of simple, rapid, cost-effective and robust on-site screening or remote quantitative analysis for ZEN, fulfilling the currently strictest regulation requirements for ZEN monitoring worldwide.
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