Abstract
A simple, easy, fast, inexpensive, and sensitive reversed phase HPLC method is developed for quantification of etoposide in plasma and biological samples. Separations are carried out using mobile phase consisting of acetonitrile and water (35:65, v/v) on phoenix C18 chromolith reverse phase analytical column protected by Phenomenex guard cartridge. Etoposide and teniposide (internal standard) are extracted from tissue samples with acetonitrile and chloroform. The eluent is monitored at 254 nm at the temperature of 45°C. The developed method exhibited linearity over an analytical range of the respective samples. Run time is only 5 min using flow rate of 1.5 mL/min with great precision and reproducibility. The peak-height ratio of etoposide with respect to internal standard is linear, corresponding to its plasma concentration. The method was reliable as both intra-day and inter-day coefficient of variation was below 5%. It is observed that this method is sensitive and reliable. The described method will be applied toward etoposide pharmacokinetic and compartmental studies in mice.
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