Rapid and sensitive detection of Staphylococcus aureus and Klebsiella pneumonia based on bacitracin-modified Fe3O4@PDA magnetic beads combined with matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Abstract

The highly effective detection of pathogens in complex biological samples is an attractive and critical topic of study. Bacitracin is a novel broad-spectrum antimicrobial peptide to enrich bacteria via interactions with the membrane surface of the different bacterial cells. In this study, for the first time, bacitracin was immobilized on the surface of Fe3O4@PDA magnetic nanoparticles for the enrichment of Staphylococcus aureus (G+) and Klebsiella pneumoniae (G-). Combined with matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a rapid and sensitive detection method for these two bacteria was developed. In this method, the detectable concentration of bacteria was decreased by 2-3 orders of magnitude in unenriched samples. The enrichment and identification can be completed in one hour. All these results demonstrated that the bacitracin-functionalized magnetic composite has potential for use in the large-scale enrichment and isolation of target pathogens from complex biological samples, opening a new avenue for the rapid and sensitive detection of pathogens.