Abstract
A rapid and sensitive CZE (Capillary Zone Electrophoresis) method for pharmaceutical analysis was developed and fully validated. The active compounds: Pseudoephedrine hydrochloride (PSE), Triprolidine hydrochloride (TRI) and Paracetamol (PAR) were separated and quantitatively determined using the tris-borate 30 mM buffer at pH = 9.0 as a Background Electrolyte (BGE). The electrophoretic separation was carried out at 25 kV in an unmodified fused silica capillary of I.D. = 50 μm with a “bubble-cell” for UV detection at 210 nm and 25°C. The separation was reached in about 3 min. After calibration the method was applied for analysis of three commercially available pharmaceutical preparations. The repeatability (RSD%) of migration time (tm) was ranging between 0.47% and 0.90% and of peak areas (A) between 0.63% and 3.64%. The Limit of Detection (LOD) values was of 0.19 μg/mL, 0.31 μg/mL and 0.08 μg/mL for respectively PSE, TRI and PAR. The results obtained in this study showed that the proposed method was useful in routinely analysis of pharmaceuticals.
Highlights
The Quality Control in pharmaceutical industry needs new reliable analytical methods able to analyse the activeHow to cite this paper: Di Berardino, S. and Jasionowska, R. (2014) Rapid and Sensitive CZE Method for Quality Control Analysis of Pharmaceuticals Containing Pseudoephedrine, Triprolidine and Paracetamol
The composite tablets containing Pseudoephedrine hydrochloride (PSE), Triprolidine hydrochloride (TRI), and PAR are commonly used for influenzal disease
The spectrophotometric determination was used for these purposes successfully [1]-[3]
Summary
The Quality Control in pharmaceutical industry needs new reliable analytical methods able to analyse the activeHow to cite this paper: Di Berardino, S. and Jasionowska, R. (2014) Rapid and Sensitive CZE Method for Quality Control Analysis of Pharmaceuticals Containing Pseudoephedrine, Triprolidine and Paracetamol. (2014) Rapid and Sensitive CZE Method for Quality Control Analysis of Pharmaceuticals Containing Pseudoephedrine, Triprolidine and Paracetamol. Many analytical methods are being developed for determination of these compounds present in various preparations as individual component or in presence of other compounds. Three papers describe the simultaneous HPLC analysis of active ingredients of our interest: PAR, PSE and TRI in pharmaceuticals [13]-[15]. CZE separation and determination of PSE, PAR and dextromethorphan in composite tablets is described [16]. The PSE present in the mixture of other seven drugs, was successfully separated and quantitatively determined by CZE method [17]. It seems useful to consider the possibility of develop a capillary electrophoretic method for routine analysis of pharmaceuticals containing PAR, PSE and TRI, as alternative of HPLC
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