Abstract
Purpose: To develop and validate an accurate, sensitive and reproducible high performance liquid chromatographic (HPLC) method for the quantitation of vitamin C in pharmaceutical samples.Method: The drug and the standard were eluted from Superspher RP-18 (250 mm x 4.6 mm, 10im particle size) at 20 0C. The mobile phase was prepared by carefully adding acetic acid (500 ml) to 1.5g of 1-hexanesulfonic acid sodium salt and mixing well (pH 2.6). The flow rate was 0.7 mL min-1. UV detector, set at 280 nm, was used to monitor the effluent.Results: Each analysis required no longer than 4 min. The limit of quantitation was 1.95 ig mL-1. Recovery (%) for different concentrations ranged from 99.58 to 101.93.Conclusion: The simplicity of this low-cost, rapid technique and its high specificity to ascorbic acid, even in the presence of a variety of excipients, demonstrate that this HPLC method would be particularlysuitable for the determination of ascorbic acid in the investigated preparations as well as other similar pharmaceutical/veterinary formulations without prior sample preparation.
Highlights
Vitamin C is a water-soluble organic compound involved in many biological processes [1]
The data indicate an adequate percentage of accuracy/recovery for the high performance liquid chromatographic (HPLC) method for the quantitation of ascorbic acid in the pharmaceutical preparations [17,18]
The specificity of the HPLC method for vitamin C quantitation in the pharmaceutical formulations is an indication of possible interference from excipients in the preparations
Summary
Vitamin C (ascorbic acid, ascorbate, AA) is a water-soluble organic compound involved in many biological processes [1]. The purpose of this study, was to develop and validate a HPLC method for the quantitation of vitamin C (ascorbic acid) in pharmaceutical powder or tablet preparations containing various excipients, without prior sample preparation. 40, 50 and 60 mg of standard ascorbic acid were weighed precisely and dissolved, separately, in 50mL of the mobile phase.
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