Abstract
"Rapid and Practical Screening Method for the Detection of Colistin-Resistant Bacteria in Food"
Highlights
Colistin is recognized as one of the few remaining available antibiotics for the treatment of intractable infections caused by multidrug-resistant Gram-negative bacteria [1]
The results indicated the potential of this method for the convenient and rapid screening of food items to detect contamination with mcr-1–positive bacteria, which can be especially useful for on-site testing in developing countries
The lower limit of mcr-1-E. coli detection for the entire method, from DNA extraction to detection by real-time polymerase chain reaction (PCR), was 7 × CFU/g; a minimum of 7 × CFU/g was required for quantification using a linear correlation
Summary
Colistin is recognized as one of the few remaining available antibiotics for the treatment of intractable infections caused by multidrug-resistant Gram-negative bacteria [1]. Rapid detection of colistin resistance genes at the research level is possible using the SYBR green method [9], but its widespread practicality is limited due to the need for complex steps and equipment involved in DNA extraction from samples and determination of result specificity. To overcome this limitation, we here report a simple, rapid, and practical detection method of Escherichia coli harboring mcr-1, as a representative CR bacterium, using a highspeed real-time polymerase chain reaction (PCR) kit. The colistin minimum inhibitory concentration (MIC) was estimated, and colistin resistance genes (mcr-1 to -5) were detected by multiplex PCR as described previously [6,11]
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