Abstract
Using flame atomic absorption spectrometry with discrete nebulization, zinc, copper, iron and magnesium were measured in a total of 100μl of serum after the four-fold dilution with Triton X-100 without removal of proteins. The final concentration of the detergent was 0.02%. For magnesium measurement, the sample solution was further diluted five times with blank solution. Every measurement was carried out with duplicate injections of 50μl of the sample solution. Standards for calibration were similarly prepared from Auto Cal 1 for matrix matching. Present method enabled the analysis of 90 samples/h without any loss in accuracy or reproducibility.
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