Abstract
Azodicarbonamide (ADA), a dough conditioner and bleaching agent of flour, is widely applied as a new flour fortifier in some certain countries. However, it is banned in Australia and Europe because of its toxicity and asthma risk for human beings. Surface-enhanced Raman spectroscopy (SERS) with ultrahigh sensitivity can offer the intrinsic fingerprint information of trace sample. Herein, based on the synthesis of the silver-coated gold nanoparticles (Au@Ag NPs), using a portable Raman spectrometer, a simple, rapid, ultrasensitive and inexpensive approach for label-free SERS detection of azodicarbonamide in flour and flour product is reported. The limits of detection (LOD) for azodicarbonamide in water, flour, steamed bread are 0.1μM (11.6ppb), 10μM (1.16ppm) and 20μM (2.32ppm), respectively, which are below the FDA's tolerance level of 45ppm in flour. Therefore, this SERS-based method of azodicarbonamide detection offers great practical potential for the effective on-site assessments of food safety.
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