Abstract
An analytical method using high performance liquid chromatography with fluorescence detection for the simultaneous determination of abamectin and ivermectin in bovine milk was developed and validated. The best recovery results were achieved by using acetonitrile for extraction of the compounds followed by solid phase extraction in cartridges containing C18 for the purification of the extract. Pre-column derivatization was accomplished with N-methylimidazole and trifluoroacetic anhydride. The method limit of detection (LOD) values for abamectin and ivermectin were 0.10 and 0.14 µg L-1 and the limit of quantification (LOQ) values were 0.18 and 0.36 µg L-1, respectively. The recoveries were from 75 to 101%, with RSD values lower than 10%. The LOD and LOQ values are lower than the maximum residue limits (MRLs) in milk established by Codex Alimentarius, European Union and the Brazilian legislation.
Highlights
The occurrence of drug residues in food and foodstuffs originating from veterinary treatments has become increasingly noticeable
Milk is known as a nutritious wholesome food consumed globally and it is an inexpensive source of protein and calcium essential for promoting growth in children and the general good health of population.[1]
Solutions with concentrations of 1, 2, 4, 6, 8 and 10 μg L-1 for abamectin and 2, 4, 8, 12, 16 and 20 μg L-1 for ivermectin were injected in triplicate
Summary
The occurrence of drug residues in food and foodstuffs originating from veterinary treatments has become increasingly noticeable. Milk is known as a nutritious wholesome food consumed globally and it is an inexpensive source of protein and calcium essential for promoting growth in children and the general good health of population.[1]. Industrial development has led to the discovery of new chemical groups, including avermectins. Abamectin was developed as an insecticide and acaricide for contact and stomach action and is highly effective against gastrointestinal nematodes of cattle,[4,5] but is not approved for use in dairy animals.[6]. Ivermectin is derived from abamectin and consists of a mixture of two homologues: 5-O-dimethyl-22,23-
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