Abstract
An apparatus for rapid determination of urea in urine was developed by combining a system for the chemical decomposition of urea with the apparatus previously developed by the present authors for blood gas analysis. In this method, urea injected into the stream of sodium nitrite-nitric acid solution was decomposed to nitrogen and carbon dioxide. Evolved gases were separated from the solution by permeating them through a silicone tube and introduced to the gas chromatograph with the carrier gas. Amount of urea was determined from peak areas of nitrogen and/or carbon dioxide in the chromatogram. When 100 μl of urine was injected, urea was determined within 7 min with coefficient of variation in less than 2 %. The precision of the present method was comparable to that by urease-indophenol method which has been conventionally used for urea determination. The correlation between the both methods was good.
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