Abstract
Semiliquidambar cathayensis Chang was a traditional medicinal plant and used to treat rheumatism arthritis and rheumatic arthritis for centuries in China with no scientific validation, while only 15 components were reported. Thus, a rapid, efficient, and precise method based on ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was applied in both positive- and negative-ion modes to rapidly analysis the main chemical compositions in S. cathayensis for the first time. Finally, a total of 85 chemical compositions, including 35 alkaloids, 12 flavonoids, 7 terpenoids, 5 phenylpropanoids, 9 fatty acids, 7 cyclic peptides, and 10 others were identified or tentatively characterized in the roots of S. cathayensis based on the accurate mass within 5 ppm error. Moreover, alkaloid, flavonoid, phenylpropanoid, and cyclic peptide were reported from S. cathayensis for the first time. This rapid and sensitive method was highly useful to comprehend the chemical compositions and will provide scientific basis for further study on the material basis, mechanism and clinical application of S. cathayensis roots.
Highlights
Semiliquidambar cathayensis Chang, is an epiphyllum tree belonging to the Hamamelidaceae family, native only to China, and grows in Jiangxi, Guangxi, Guangdong, Hainan and Guizhou [1]
A series were of parameters, including stationary mobile phases, flow rate, and column temperature investigated in order to obtainand optimal chromatographic separation andtemperature analytical were investigated in order to obtain optimal chromatographic separation and analytical sensitivity for sensitivity for multiple constituents in the roots of S. cathayensis
According to reference mass spectra and fragmentation spectra reported in the literatures [46,51,52], a total of seven cyclic peptides were identified with 3–9 leucyl groups in the roots of S. cathayensis
Summary
Semiliquidambar cathayensis Chang, is an epiphyllum tree belonging to the Hamamelidaceae family, native only to China, and grows in Jiangxi, Guangxi, Guangdong, Hainan and Guizhou [1]. Due to the indeterminate relationship between pharmacological activities, chemical components, the clinical application and Molecules 2019, 24, 4098; doi:10.3390/molecules24224098 www.mdpi.com/journal/molecules fatty acids—were reported from the roots of S. cathayensis [7,8], which was a significant barrier for further pharmacological, metabolic and pharmacokinetic studies of this medicinal plant. Quadrupole time-of-flight Q-TOF-MS/MS with powerful of its advantages on the peak capacity, resolution, separation time, and detection sensitivity, all of which are structural characterization can provide more specific and accurate mass measurements for both suitable for addressing the complicated characteristics of the constituents in TCMs [14,15,16,17]. These features can greatly facilitate prediction of elemental quadrupole time-of-flight Q-TOF-MS/MS with powerful structural characterization can provide more compositions and fragmentation pathways [18,19,20]. The information of potential new drug structure for treating rheumatism arthritis and rheumatic arthritis
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