Abstract
The distribution and content differentiation of morusin in the cultivated species of mulberry by HPLC-DAD are described in this paper. The experimental results showed that morusin is present in all parts of the mulberry bush. The content of morusin was highest in root bark and second highest in branch bark. The difference in morusin content of 20 different species of cultivated mulberry branch bark was significant. The level of morusin was highest in the branch bark of cultivated mulberry No. 404, a tetraploid cultivar, and third in the Husang 32 cultivar of Morus multicaulis. The method used in this study for determining morusin content exhibited good repeatability (RSD 6.02%) and recovery (100.62%). Therefore, the results from this study provide reliable data, for research and development in the future, on the level and distribution of morusin in mulberry in general and the differences found between various cultivated mulberries in particular. Furthermore, the HPLC-DAD method to determine morusin content is fast and reliable and is applicable not only to mulberry bushes but also to other plants.
Highlights
Morus (Moraceae) is a small genus found in the temperate and subtropical regions of the northern hemisphere
The results show that the peak UV absorption spectrum of the branch bark ethanol extract of Husang 32 was almost the same as the standard morusin sample with
Husang 32 and the branch bark from 20 other cultivated species of mulberry were used as experimental material
Summary
Morus (Moraceae) is a small genus found in the temperate and subtropical regions of the northern hemisphere. The silk industry countries, such as Japan, China, North Korea, South Korea, Thailand and Brazil cultivate mulberry bushes on a large scale. In China, the mulberry tree has been widely cultivated and the different components of the bush have been used in traditional Chinese medicine for thousands of years. The mulberry leaves, which are an important food for silkworm, have been found to have pharmacological benefits with regards to hyperglycemia and hyperlipidemia [1,2,3] and have an antioxidant action [4]. The fruit is an antioxidant and tonic agent [5]. The root bark, called “Sang-Bai-Pi” in Chinese, is considered an important anti-inflammatory [6], antidiabetic [7], antitumor [8] and antinephritis [9] medicine
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