Rapid Analysis of Animal Drug Residues by Microcolumn Solid-Phase Extraction and Thermal Desorption–Ion TYap Mass Spectrometry

Abstract

A new approach was developed for the rapid and quantitative determination of an anthelmintic drug, phenothiazine, in milk. The technique involves a simple extraction procedure using a C18 microcolumn disc, followed by thermal desorption of the analyte from the disc directly into an ion trap mass spectrometer. The compounds are selectively ionized by isobutane chemical ionization and detected by tandem mass spectrometry. With this approach, 10 ppb detection limits were achieved with as little as 100 microL milk and only 10 min of analysis time. This approach was used to analyze samples of milk taken from a cow administered a one-time therapeutic dose of phenothiazine. The target compound could be detected at 56 h post-dosage, corresponding to a concentration of 30 ppb.