Abstract
We present a polymer-enhanced capillary transient isotachophoresis (PectI) selection methodology for acquisition of high-affinity (kinetically inert) DNA aptamers capable of recognizing distinct microbial cell surfaces, which requires only a single electrophoretic separation between particles (free cells and cells bound with aptamers) and molecules (unbound or dissociated DNA) in free solution.
Highlights
Rapid acquisition of high-affinity DNA aptamer motifs recognizing microbial cell surfaces using polymer-enhanced capillary transient isotachophoresis
We present a polymer-enhanced capillary transient isotachophoresis (PectI) selection methodology for acquisition of high-affinity DNA aptamers capable of recognizing distinct microbial cell surfaces, which requires only a single electrophoretic separation between particles and molecules in free solution
Whereas the analysis of bacterial cells by conventional capillary electrophoresis (CE) typically results in the appearance of multiple peaks with very low repeatability, we have recently developed a CE-based separation mode for bacterial cells, called ‘‘Polymer-enhanced capillary transient Isotachophoresis (PectI)’’, which yields a single peak per cell type with high repeatability.[3]
Summary
Rapid acquisition of high-affinity DNA aptamer motifs recognizing microbial cell surfaces using polymer-enhanced capillary transient isotachophoresis. We present a polymer-enhanced capillary transient isotachophoresis (PectI) selection methodology for acquisition of high-affinity DNA aptamers capable of recognizing distinct microbial cell surfaces, which requires only a single electrophoretic separation between particles and molecules in free solution.
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