利用形態性狀及RAPD、ISSR分子標誌探討馬祖東引地區換錦石蒜（Lycoris sprengeri）種原歧異度

Abstract

The purpose of this research was discussing genetic diversities within groups and between groups of four habitants of ＂Lycoris sprengeri＂ native in Tung-yin Township in Ma-tzu. Through collection of genetic resource and phenotype data of ＂Lycoris sprengeri＂ in Tung-yin, the relationship between individuals in different habitants was figured and useful for the following work of restoration and breeding. The investigation included morphological and molecular marker analysis. Phenotypes included length of scape and petal, width of petal, floret number, size of floret and cyme. In molecular marker analysis, DNA samples of ＂Lycoris sprengeri＂ collected from 4 habitats was analyzed with 15 RAPD markers and 6 ISSR markers. Through genetic similarity matrix and Unweighted Pair Group Method with Arithmetic Mean conducted by NT-SYS, the genetic diversity of ＂Lycoris sprengeri＂ in Tung-yin was estimated by similarity tree and principle coordinates analysis results. As a result, there were significant differences in length of scape, length of petal and diameter of cyme between individuals of the same color types in different habitants. On the other hand, the similarity coefficiency of the collected samples was ranged in 0.64-0.85, and there was no obvious grouping among individuals in the same habitats. However, diversities was existed in each habitats. The results benefited ＂Lycoris sprengeri＂ adapting to environmental changes and work of restoration and breeding program we want. It is worth to preserve the diversely genetic resources in the small island that could be considered as secondary center of diversity.