Abstract
In this paper we reported some genetic characteristics in Cucumis melo based on the analysis of random amplified polymorphic DNA (RAPD) markers using a 3-generation population. It has been found in this study that the variation of RAPD banding patterns will occur if restriction enzyme was used for template DNA digestion before PCR. By the use of enzyme, the band number can be either reduced or increased. Our results showed that those phenomena can be stably repeated. This method can significantly enhance the efficiency of RAPD analysis. Some hypotheses were proposed to interpret the mechanism causing these phenomena and for the further understanding of RAPD markers. Our investigation has also confirmed the stability and independent behavior of RAPD-loci. Some genetic characteristics of C. melo have also been discussed based on the RAPD analysis.
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