Abstract
RAPD-PCR and UP-PCR analysis allowed us to differentiate Verticillium lecanii strains isolated from insect (Homoptera) and from rust fungi. UPGMA, NJ and NJ consensus trees revealed groups of isolates according to their geographical origin. Successful amplification of the ITS region occurred in a few V. lecanii strains. These ITS sequences were almost identical to known ITS sequences. Thirteen strains had similar IGS sequence of about 2.2 Kb, while strain V5 had a different and shorter (1.1 Kb) IGS-sequence. Among the isolates the correlation between conidia size and virulence towards whitefly was low. Verticillium lecanii strains were able to prevent the pustule development of Puccinia recondita. V61 showed 98% effectiveness. Strains active against wheat rust causal agent were highly virulent against whitefly and show some antibacterial activity. These strains are promising for the biocontrol of plant diseases.
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