Abstract
Single, short generic primers of arbitrary sequence used to amplify DNA from Penaeus monodon gave three polymorphic markers out of the total of 48 bands that amplified. This level of polymorphism is similar to that displayed by other taxa (6–7%) and suggests randomly amplified polymorphic DNA (RAPD) approaches will be as useful in providing markers for prawn breeding programs as they have been for other species. Fifty offspring from each of the six families screened for the three polymorphic markers demonstrated that all the markers conformed to Mendelian expectations for band presence/absence, consistent with a model of complete or co-dominance where dominant homozygotes and heterozygotes display a band but homozygote recessives do not. A fourth primer amplified a band in almost all of the progeny but in none of the adults, and was probably derived from algal or bacterial epicommensals contaminating the sample, emphasising the need to establish the inheritance of RAPD polymorphisms prior to their use as genetic markers.
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