RAPD MARKER TO SCREENING GENETIC DIVERSITY OF LOCAL CHICKEN

Abstract

A total of 500 local chickens from different regions of Iraq, which were divided into five groups according to the regions from which they were taken, namely, the East (E), West (W), North (N), South (S), and Central (C) regions of Iraq. The blood samples were collected into tubes containing an EDTA, DNA extraction was carried. Using 20 primers from Gen Script USA, random amplification of polymorphic DNA (RAPD-PCR) was carried out. The reaction of PCR had a final volume of 25 μl. A total of 473 loci ranging between 200 and 1800 bp were amplified. The higher numbers of bands shown in north(N) local chicken group (101) than the other groups. East (E) and South (S) groups phenotypic revealed higher numbers of the polymorphic bands. The Polymorphism percent (%6.2) in East (E). Between the north (N) and west (W) groups, the genetic similarity score was found to be the highest (0.893). dendogram It was clear that the South(S) appeared to be most distant from the other groups whereas the West (W), North (N) were related closely together whose genetic closeness is highest. The main aim of present study is to assess the genetic specificity chickens in Iraq independs on RAPD analysis, To assess phylogenetic relatedness between groups of chickens, is to understand the extent of genetic variation between local chickens groups in Iraq.