Abstract

A genetic linkage map of Brassica carinata (BBCC; 2n = 34) has been constructed with 23 RAPD, 29 ISSR and 17 SSR markers using an F2 hybrid population of 150 individuals. The mapping population was developed by selfing single F1 plant following hybridization between a resynthesized B. carinata genotype, Ar29 and natural B. carinata cv. PC5. The segregation of each marker and linkage analysis was performed using the program MapMaker v3.0. The 69 mapped markers were aligned into 17 major linkage groups and a subgroup 5A at LOD value of 3.0. The total length of the map was 2,166 cM, with an average marker interval of 31.39 cM. To the best of our knowledge, this is the first report on genetic linkage mapping of B. carinata and the map developed in the present study will be highly useful for anchoring of more loci in future investigations.

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