Abstract
Background: Soybean is one of the most important commodities around the globe. Increasing soybean production absolutely continue to be pursued because soybean consumption increased steadily. One way that can be done is assembly technology of new superior varieties that have better and adaptive properties. The aim of this study was to determine molecular diversity of soybean mutants lines resulting from gamma irradiation in M6 generation. Methods: Molecular analysis with RAPD markers was carried out in the Tropical Balitbu Laboratory Sumatera Barat, Indonesia in November 2021. The tested genotypes consisted of 8 genotypes, namely Kipas Putih variety (elderly), Anjasmoro variety (comparison), M.1.1.3, M.5.2.1, M.5.2.3 l, M.1.1.8, M.1.1.9 and M.1.1.17 mutant lines. The primers used were RAPD 2 (5´-GTTTCGCTCC-3´), RAPD. 3 (5´-GTAGACCCGT-3´), RAPD 4 (5´-AAGAGCCCGT-3´) and RAPD 5 (5´-AACGCGCAAC-3´). Result: The results of the RAPD analysis showed that there were differences in size of amplicon between the mutants and the parents, namely the RAPD 2 primer (1400 bp band) and the RAPD 5 primer (550 bp and 1000 bp band). The parents had a pattern of DNA bands but in the M.5.2.3 and M.1.1.9 mutants the DNA band was absent. The parent RAPD 5 primers (1000 bp, 550 bp) had a DNA banding pattern but in the M.1.1.3 and M.1.1.8 mutants there was no DNA banding pattern. This indicated that the M.1.1.3, M.5.2.3, M.1.1.8 and M.1.1.9 mutants had different genetic diversity from their parent (Kipas Putih variety).
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.