Abstract
In this study, the RAPD (Random Amplified Polymorphic DNA) technique was employed for determination of the components in an Ayurvedic herbal prescription, Rasayana Churna. One-hundred-and-twenty decamer oligonucleotide primers were screened in the RAPD analysis to identify three Ayurvedic medicines, dried stem of Tinospora cordifolia, dried fruit of Emblica officinalis and dried fruit of Tribulus terestris, the Ayurvedic prescription. Primer OPC-6 simultaneously generated three distinct amplicons, each specific to one component. The marker with 600 bp is specific to Tinospora cordifolia; the marker 500 bp is specific to Emblica officinalis and the remaining marker >1000 bp was present in Tribulus terestris. Presence of three herbal medicines was determined when RAPD reaction with OPC-6 was performed. The technique was proved to contribute to the identification of components in Ayurvedic herbal preparation and thus helping to serve as a complementary tool for quality control.
Highlights
Herbal medicine has been enjoying renaissance among the customers throughout the world
Dried stem of T. cordifolia, fruit of E. officinalis and fruit of T. terestris were washed with 70% ethanol for 5 min and with sterile deionized water for 1 min following ethanol, using sonication to avoid surface contamination
In the present article Rasayana churna, an Ayurvedic formulation was chosen to test the reliability of the quality control and presence of three herbal drugs in it, using Random Amplified Polymorphic DNA (RAPD) technique
Summary
Herbal medicine has been enjoying renaissance among the customers throughout the world. Due to the complex nature and inherent variability of the chemical constituents of plant-based drugs, it is difficult to establish quality control parameters [1]. Rasayana churna is an Ayurvedic polyherbal formulation used for its antioxidant, immunomodulatory and rejuvenating purpose. It includes three herbal drugs, dried stem of Tinospora cordifolia (family: Menispermiaceae), dried fruit of Emblica officinalis (family:Euphobiaceae) and dried fruit of Tribulus terestris (family:Zygophylaceae), in powder form [2]. Random Amplified Polymorphic DNA (RAPD) involves the use of a single ‘arbitrary’ primer in a polymerase chain reaction (PCR) and results in the amplification of several discrete DNA products [3]. The assay will be helpful in quality control of herbal medicines in the market
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