Abstract
A new nothospecies Ranunculus ×prieti is described following morphological and molecular characterization. Hypervariable Amplified Fragment Length Polymorphism (AFLP) markers, flow cytometry (FCM) analysis and pollen viability were conducted. The profiles of hybrid samples were compared to their presumed parental taxa R. amplexicaulis and R. gramineus. Morphological study revealed that all hybrid individuals were morphologically intermediate between their parents, showing ovate-lanceolate basal leaves and amplexicaul cauline leaves as in R. amplexicaulis and yellow corollas as in R. gramineus. Principal coordinates analysis of the AFLP data set showed that hybrids are closer to R. gramineus than to R. amplexicaulis, whereas the results obtained by flow cytometry indicate otherwise. Pollen fertility estimation of R. ×prieti ranged from 68.83 to 95.81%, showing that hybrid samples are well established and fertile.
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