RANKL-binding peptide promotes ectopic bone formation induced by BMP-2 gene transfer in murine gastrocnemius muscle

Shigeki Nagahiro,Toshimi Sato,Shingo Sato,Takashi Ono,Mariko Yamamoto Kawai,Michiyo Miyashin,Tomoki Uehara,Preksa Keo,Shinji Kuroda,Kazuhiro Aoki,Hiroki Ochi

doi:10.15761/domr.1000327

Abstract

RANKL-binding peptide promotes ectopic bone formation induced by BMP-2 gene transfer in murine gastrocnemius muscle

Highlights

Bone morphogenetic protein-2 (BMP-2), a bone-formation inducer, has been widely used in bone regeneration [1]
No significant increase in alkaline phosphatase (ALP) activity was noted in the BMP-2-genetransfected wells compared to the vehicle control (0 μM W9), an increase was observed in the presence of 100 μM of W9 in the BMP2-gene-transfected ST2 cells
The present study revealed the beneficial effects of RANKLbinding peptide on bone formation and the quality of bone induced by BMP-2 gene transfer

Summary

Introduction

Bone morphogenetic protein-2 (BMP-2), a bone-formation inducer, has been widely used in bone regeneration [1]. The direct injection of genetic materials, such as via a plasmid vector, can be performed to induce bone formation without using a scaffold material, since gene-transfected cells can release the protein to the scaffold materials, releasing the protein sustainably over a given duration [2]. Various routes for BMP-2 gene transfer have been developed, with two kinds of vectors mainly used: non-viral and viral vectors [1]. Despite the development of a number of other methods for increasing the amount of bone induced using non-viral vectors, such as sonoporation (cell sonication) [9] and cationic polymers [1,10], the amount of bone induced remains insufficient for clinical applications

Methods

Results

Conclusion