Abstract
RAPD is a PCR based technique which involves the use of single arbitrary short primers (8-12 nucleotides), resulting in the amplification of many discrete DNA. The segments of DNA that are amplified are random. The technique was developed independently by two different laboratories and called as RAPD and AP-PCR (Arbitrary Primed PCR). This procedure detects nucleotide sequence polymorphisms in a DNA amplification based assay using only a single primer of arbitrary nucleotide sequence. The RAPD technology has provided a quick and efficient screen for DNA-sequence polymorphisms at a very large no of loci. The present communication gives emphasis on basic knowledge about RAPD, procedure, its advantages disadvantages, limitations and applications of RAPD.
Highlights
Replication to ensure that only reproducible bands are scored
RAPD is a Polymerase Chain Reaction (PCR) based technique which involves the use of single arbitrary short primers (8-12 nucleotides), resulting in the amplification of many discrete DNA
The results indicated that RAPD could be effectively used for genetic diversity analysis in wild species of prospective value as it is reliable, rapid and superior to those based on pedigree information Raghunathachari et al (2000) studied a set of set of 18 accessions from an Indian scented rice (Oryza sativa L.) and they were subjected to random amplified polymorphic DNA (RAPD) analysis
Summary
Replication to ensure that only reproducible bands are scored. RAPD analysis has been extensively used for Estimation of genetic variations are increasingly various purposes which include identification and being based upon information at the DNA level by classification of accessions (Fukuoka et al, 1992), various molecular techniques such as Randomly identification of breeds (Qian et al, 1996) and genetic. The results indicated that RAPD could be effectively used for genetic diversity analysis in wild species of prospective value as it is reliable, rapid and superior to those based on pedigree information Raghunathachari et al (2000) studied a set of set of 18 accessions from an Indian scented rice (Oryza sativa L.) and they were subjected to random amplified polymorphic DNA (RAPD) analysis. In a study by Muz et al (2005) 30, 15 and 1 strains of Pasteurella multocida and 9, 8 and 6 strains of Mannheimia haemolytica from cattle, sheep and goats isolated in Elazig province located in the East of Turkey, respectively were typed by Random Amplified Polymorphic DNA (RAPD) assay using a random primer (OPA-11). Rapd is a new technique based on PCR (Williams et al, 1990; Welsh and McClelland, 1990) It is a convenient, efficient and sensitive genetic marker for detecting the polymorphism of genome DNA. Efficient and sensitive genetic marker for detecting the polymorphism of genome DNA. 10 base (GC-rich) oligonucleotide primers of arbitrary sequence were used in the PCR amplification and it can get amplification results of several loci
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