Random laser marked PLCD1 gene therapy effect on human breast cancer

Abstract

The utilization of laser spectroscopy has seen a dramatic increase in the past several decades and applications in experimental and theoretical development can be found at present in such areas as biomolecular research and medical practice. To verify the feasibility of a random sensitive means for marking the PLCD1 gene therapy effect on breast cancer, in this paper, the random lasing properties were studied in two groups of human breast xenograft tissues. Both coherent and incoherent random lasing regimes were observed from the AdHu5-EGFP group and the AdHu5-PLCD1 group, respectively. A question remains whether the tumor tissue sample receives PLCD1 gene therapy, and this issue becomes critical to the sample’s lasing spectrum performance. The inherent disorder of tumor breast tissues produces more light scatters randomly distributed in the tumor sample, resulting in coherent random lasing emission. Indeed, hematoxylin and eosin staining images attest to the fact that the spatial arrangement of the cells of the breast tumor exhibits more disorder and their organizational structure exhibits more irregularity than that of tumor tissues that receive PLCD1 therapy. Our studies pave the way for a random laser marked gene therapy for breast cancer.