Abstract
Development of a simple, accurate, and rapid method for identifying malting barley cultivars is important for the malting, brewing, and seed-processing industries. Recently reported methods that have been developed for using DNA to “fingerprint” barley utilize DNA that is extracted from leaf tissue. For this study, we used the polymerase chain reaction-random amplified polymorphic DNA (PCR-RAPD) technique with a selected set of 10-mer primers and DNA that was extracted from mature imbibed embryos. We were able to differentiate 16 malting barley cultivars or breeding lines that are commonly grown in North America, including the two-rowed cultivars Crystal, Garnet, Galena, Harrington, and B1202 and the six-rowed cultivars Robust, Stander, Morex, Excel, Lacey, Foster, Drummond, Russell, 88Ab536-B, B2601, and B2978. This method is simple to use and can be accomplished in 12–16 hr, since it bypasses the time-consuming germination and seedling growth steps. PCR results using embryo DNA samples are comparable to those obtained with leaf tissue DNA. The rapid and simple procedure that we have developed can be adapted by industry to maintain cultivar purity and to check the integrity of purchased seed lots.
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