Random amplified polymorphic DNA (RAPD) typing of necrophageous insects (Diptera, Coleoptera) in criminal forensic studies: validation and use in practice

Abstract

To permit quick identification of arthropods, random amplified polymorphic DNA typing (RAPD) was used to support classical morphological and medico-legal analysis of maggots on a human corpse. The method was employed to determine if maggots which were found on the inside of a body bag were identical (a) with maggots found on the outside of the bag, and (b) pupae found on the floor under the corpse. Pre-mixed RAPD reaction beads together with semiautomatic computer aided analysis of the PCR products are shown to discriminate between closely related necrophageous insect species (flies and beetles) found on corpses. From the 11 RAPD primers used, one alone was sufficient in resolving a practical forensic situation. This is the first report of a forensic application of RAPD DNA typing.