Abstract
Raman spectroscopic data have been obtained with the use of direct current (dc) signal detection, an IBM PC/AT microcomputer, and commercially available software. Since photomultiplier currents of the order of nanoamps to microamps are readily attained for Raman emission under conditions of moderate laser excitation power levels (150–200 mW) and medium resolution spectral slits (1–4 cm−1), signal levels well within the domain measurable by dc signal detection techniques are achieved for a wide range of chemical and biochemical samples. Further, the digitization and signal averaging capabilities of generic data acquisition boards and microcomputers allow dc detection to yield signal-to-noise ratios competitive with those derived from complementary pulse-counting techniques.
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