Abstract

Raman spectroscopy plays a major role in robust detection of biomolecules and mineral signatures in halophile research. An overview of Raman spectroscopic investigations in halophile research of the last decade is given here to show advantages of the approach, progress made as well as limits of the technique. Raman spectroscopy is an excellent tool to monitor and identify microbial pigments and other biomolecules in extant and extinct halophile biomass. Studies of bottom gypsum crusts from salterns, native evaporitic sediments, halite inclusions, and endoliths as well as cultures of halophilic microorganisms permitted to understand the content, distribution, and behavior of important molecular species. The first papers describing Raman spectroscopic detection of microbiological and geochemical key markers using portable instruments are highlighted as well.

Highlights

  • Laser Raman spectroscopy determines the wavelength and strength of inelastically dispersed light from molecules and their arrangement

  • Raman spectroscopy plays a major role in robust detection of biomolecules and mineral signatures in halophile research

  • The information gained was used to characterize natural communities of halophilic microorganisms in saltern brines and of halophiles trapped in fluid inclusions within halite crystals, recent as well as ancient

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Summary

Introduction

Laser Raman spectroscopy determines the wavelength and strength of inelastically dispersed light from molecules and their arrangement. Raman spectroscopy studies of pigmented halophiles in culture, have included red archaea of the family Halobacteriaceae, the β-carotene-rich alga Dunaliella salina, and Salinibacter ruber, a member of the phylum Bacteroidetes.

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