Abstract

Fats and oils are ubiquitous in natural and processed foods, providing necessary energy storage. Fat and oil content in foods also have important contributions to the shelf life, texture, compatibility with processing operations, and sensory profiles of food products. Understanding the molecular properties of fats and oils separately within a heterogeneous food matrix requires a multidisciplinary approach. Vibrational spectroscopy techniques are used throughout the food industry to gain product understanding, identify adulterated products, ensure quality, and control processes. In analyzing fats and oils in food, near-infrared (NIR) spectroscopy is an established analytical technique, and there are other growing applications of infrared (IR) and Raman spectroscopies. In particular, Raman spectroscopy is well suited to measure fats and oils because their C-H and C-C bonds are polarizable. In this article, we review the historical use of Raman spectroscopy in studying fats and oils in foods from Fourier transform (FT)–Raman spectroscopy to dispersive Raman spectroscopy. We also provide an overview of various Raman approaches to understand fat compositional heterogeneity in solid foods, identify polymorph or crystallinity, and measure fatty acid saturation. Examples in a variety of fat-containing foods demonstrate feasibility for Raman applications in the laboratory and process environments.

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