Abstract
In this study, we utilized a stainless steel (SUS304) plate for measuring the Raman scattering spectra of body fluid samples. Using this stainless steel plate, we recorded the Raman scattering spectra of 99.5% ethanol and human serum samples by performing irradiation with 785- and 1064-nm lasers. Raman scattering spectra with intensities equal to or greater than those reported previously were obtained. In addition, the Raman scattering spectra acquired using the 1064-nm laser were less influenced by autofluorescence than those obtained via use of the shorter-wavelength laser. Moreover, the shapes of the spectra did not show any dependence on integration time, and denaturation of the samples was minimal. Our method, based on 1064-nm laser and the stainless steel plate, provides performance equal to or better than the methods reported thus far for the measurement of Raman scattering spectra from liquid samples. This method can be employed to rapidly evaluate the components of serum in liquid form without using surface-enhanced Raman scattering.
Highlights
Raman scattering spectroscopy is a nondestructive evaluation method that can be used to investigate the component ratios and structures of materials in a noncontact manner [1]
In the detected Raman scattering spectra of ethanol (99.5%) including in the region of the bands corresponding to the intramolecular vibrations of C−C molecules at 886 cm-1, the autofluorescence from the glass was relatively strong (Fig 2) [12]
Raman scattering spectra of ethanol (99.5%) obtained using a conventional slide glass
Summary
Raman scattering spectroscopy is a nondestructive evaluation method that can be used to investigate the component ratios and structures of materials in a noncontact manner [1]. Human body fluids are analyzed using Raman spectroscopy in order to gain biochemical information for medical research and several methods for obtaining the Raman scattering spectra of human body fluids have been reported [5], but standard methods have not yet been established. Because the components of human serum are diverse and exist in very small amounts, it is difficult to obtain the Raman scattering spectra of human serum that has not been pretreated without denaturing the liquidstate serum. Autofluorescence is generated if ordinary glass is used for the slide glass, so synthetic quartz, which has weaker autofluorescence, is used; synthetic
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