Abstract
Raman spectroscopy with 1064 nm laser excitation is used here to identify the chemical composition of the extracts obtained from Pycnoporus sanguineus fungus, in comparison with the data produced from the red fungus itself. Polar and non-polar solvents were used to separate cinnabarin and ergosterol, respectively, the main components of each extract. The Raman spectra of the extracts are dominated by specific vibrational modes that can be related to these components; in the case of ergosterol the main bands are those assignable to CH stretching and deformation along with bands related to the aryl skeletal rings. The cinnabarin fraction, on the other hand, gives a Raman spectrum where the most important bands are those related to NH2 bending (1510 cm−1) and C quinonoid stretching (1647 cm−1) modes. The Raman spectrum obtained directly from the fungus shows similarity with the cinnabarin fraction, in agreement with the literature information from extracts that cinnabarin is the most significant component present in the red fungus. This result highlights the potential of Raman spectroscopic techniques for the monitoring of the fungus extraction process undertaken in small industries. Copyright © 2007 John Wiley & Sons, Ltd.
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